Open AccessBacillus salidurans sp. nov., isolated from salt-accumulated pepper rhizospheric soil
Author(s) -
JinSoo Son,
Ye-Ji Hwang,
Soo-Yeong Lee,
SaYoul Ghim
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003110
Subject(s) - biology , 16s ribosomal rna , peptidoglycan , strain (injury) , microbiology and biotechnology , diamino acid , bacteria , phylogenetic tree , pepper , bacillus (shape) , soil microbiology , botany , cell wall , gene , biochemistry , food science , genetics , anatomy , gene sequence
A Gram-stain-positive, facultatively anaerobic, motile and rod-shaped bacterium, designated KNUC7312 T , was isolated from salt-accumulated rhizospheric soil in a pepper greenhouse in Miryang city, Republic of Korea. Cell growth of strain KNUC7312 T occurred at 10-45 °C (optimum, 30 °C) and pH 7-12 (optimum, pH 7). In addition, this strain was able to tolerate 0-12 % NaCl (w/v) concentration (optimum, 0-1 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain KNUC7312 T clustered together with other species of the genus Bacillus and was most closely related to Bacillus humi DSM 16318 T (98.0 %). The predominant respiratory quinone was menaquinone-7 (MK-7). The major cellular fatty acids were anteiso C15 : 0, iso-C15 : 0 and iso-C14 : 0. The polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and two unidentified aminolipids. The cell-wall peptidoglycan contained meso-diaminopimelic acid as the major diagnostic diamino acid. Strain KNUC7312 T showed a low DNA-DNA relatedness value (47.36 %) with B. humi DSM 16318 T , which supported that this strain represents a novel Bacillusspecies. On the basis of phenotypic, chemotaxonomic and phylogenetic evidence, strain KNUC7312 T represents a novel species within the genera Bacillus. The name Bacillus salildurans sp. nov. is proposed. The type strain is KNUC7312 T (KCTC 33852 T =CGMCC 1.13629 T ).