Open AccessStreptomyces reniochalinae sp. nov. and Streptomyces diacarni sp. nov., from marine sponges
Author(s) -
Lei Li,
Jie Wang,
Yongjun Zhou,
Hou-Wen Lin,
Yanhua Lü
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003109
Subject(s) - biology , 16s ribosomal rna , streptomyces , phylogenetic tree , strain (injury) , diamino acid , actinomycetales , microbiology and biotechnology , streptomycetaceae , botany , bacteria , gene , genetics , gene sequence , anatomy
Two marine actinomycete strains, LHW50302 T and LHW51701 T , were isolated from marine sponges collected in Sansha, Hainan Province, China. The morphological, chemotaxonomic and phylogenetic characteristics were consistent with their classification in the genus Streptomyces. The strains formed hooked and looped chains of arthrospores with smooth surfaces. The cell-wall hydrolysates of the strains contained ll-diaminopimelic acid as the diagnostic diamino acid. MK-9(H8) was the predominant menaquinone. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. Major fatty acids of the strains were iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0. The 16S rRNA gene sequences indicated that the strains clustered together with Streptomyces albus CGMCC 4.1640 T and Streptomyces qinglanensis CGMCC 4.6825 T . Multilocus sequence analysis (MLSA) confirmed their relationship. Genome relatedness in forms of average nucleotide identity, digital DNA-DNA hybridization value and MLSA evolutionary distance between each of the strains and its closest relatives showed that they belonged to distinct species. On the basis of these results, strains LHW50302 T and LHW51701 T belong to two novel species in the genus Streptomyces, for which the names Streptomyces reniochalinae sp. nov. (type strain LHW50302 T =CCTCC AA 2018013 T =DSM 106194 T ) and Streptomyces diacarni sp. nov. (type strain LHW51701 T =CCTCC AA 2018017 T =DSM 106126 T ) are proposed, respectively.