Open AccessLysobacter silvisoli sp. nov., isolated from forest soil
Author(s) -
Xianjiao Zhang,
Qing Yao,
Yonghong Wang,
Song-Zhen Yang,
Guang-Da Feng,
Honghui Zhu
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003105
Subject(s) - 16s ribosomal rna , biology , phylogenetic tree , strain (injury) , phosphatidylglycerol , whole genome sequencing , bacteroidetes , botany , microbiology and biotechnology , bacteria , soil microbiology , gene , genome , genetics , phospholipid , anatomy , membrane , phosphatidylcholine
A yellow-pigmented, Gram-stain-negative, gliding and rod-shaped bacterial strain, designated zong2l5 T , was isolated from a forest soil sample at Dinghu Mountain, Guangdong Province, PR China. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain zong2l5 T belongs to the genus Lysobacter, and was most closely related to Lysobacter enzymogenes KCTC 12131 T (97.7 %) and Lysobacter soli KCTC 22011 T (97.6 %). The novel strain showed an average nucleotide identity (ANI) value of 81.5 % and a digital DNA-DNA hybridization (dDDH) value of 25.3 % with L. enzymogenes KCTC 12131 T based on draft genome sequences, followed by L. soli KCTC 22011 T with ANI and dDDH values of 79.4 % and 22.7 %, respectively. The DNA G+C content of strain zong2l5 T based on the whole genome sequence was 69.2 mol%. The major fatty acids were iso-C15 : 0, iso-C17 : 0 and summed feature 9 (iso-C17 : 1ω9c and/or 10-methyl C16 : 0). Strain zong2l5 T contained Q-8 as the major isoprenoid quinone and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidyl-N-methylethanolamine, phosphatidylethanolamine, three unidentified phospholipids and an unidentified aminolipid. The phenotypic, genotypic and chemotaxonomic anlyses clearly showed that strain zong2l5 T represents a novel species of the genus Lysobacter, for which the name Lysobactersilvisoli sp. nov. is proposed. The type strain is zong2l5 T (=GDMCC 1.1489 T =KCTC 52923 T ).