Open AccessSphingopyxis lindanitolerans sp. nov. strain WS5A3pT enriched from a pesticide disposal site
Author(s) -
Michał A. Kaminski,
Adam Sobczak,
Grzegorz Spólnik,
Witold Danikiewicz,
Andrzej Dziembowski,
Leszek Lipiński
Publication year - 2018
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003094
Subject(s) - biology , strain (injury) , pesticide , microbiology and biotechnology , ecology , anatomy
An aerobic, Gram-stain-negative, rod-shaped, non-motile, mesophilic soil bacterium, strain WS5A3p T , was isolated from a pesticide burial site in north-west Poland. The strain grew at 12-37 °C, at pH 8-9 and with 0-2 % (w/v) NaCl. The main fatty acids detected in WS5A3p T were summed feature 3, summed feature 8 and C16 : 0. The major respiratory quinone was Q-10 and major polar lipids were phosphatidylethanolamine, sphingoglycolipid and phosphatidylglycerol. The G+C content of the genome was 65.1 mol%. Phylogenetic pairwise distance analysis of the 16S rRNA gene placed this strain within the genus Sphingopyxis, with the highest similarity to Sphingopyxis witflariensis W-50 T (98.8 %), Sphingopyxis bauzanensis BZ30 T and Sphingopyxis ginsengisoli Gsoil 250 T (98.3 %) and Sphingopyxis granuli NBRC 100800 T (98.09 %). Genomic similarity analyses using ANIb and dDDH algorithms indicated levels of similarity of 81.44, 80.84 and 81.16 % between WS5A3p T and S. witflariensis, S. bauzanensisand S. granuli, respectively for average nucleotide identity and 25.90, 25.00 and 26.10 % for digital DNA-DNA hybridization. Based on the phylogenetic and phenotypic data, strain WS5A3p T should be considered as a representative of a novel Sphingopyxis species. The name Sphingopyxis lindanitolerans sp. nov. is proposed with the type strain WS5A3p T (=DSM 106274 T =PCM 2932 T ).