Open AccessEnterobacter sichuanensis sp. nov., recovered from human urine
Author(s) -
Wenjing Wu,
Yu Feng,
Zhiyong Zong
Publication year - 2018
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003089
Subject(s) - biology , enterobacter , rpob , 16s ribosomal rna , housekeeping gene , strain (injury) , phylogenetic tree , microbiology and biotechnology , genomic dna , sequence analysis , whole genome sequencing , genetics , gene , genome , escherichia coli , gene expression , anatomy
An Enterobacter strain, WCHECL1597 T , was recovered from the urine of a patient in China in 2016. Phylogenetic analysis based on its 16S rRNA gene sequence indicated the strain belongs to the genus Enterobacter, while multi-locus sequence analysis of the rpoB, gyrB, infB and atpD housekeeping genes revealed that the strain was distinct from any previously described Enterobacter species. The whole genome sequence of strain WCHECL1597 T had a 79.81-91.49 % average nucleotide identity to those of type strains of all known Enterobacter species. In silico DNA-DNA hybridization values between strain WCHECL1597 T and the type strains of all known Enterobacter species ranged from 22.8 to 45.2 %. The major cellular fatty acids of strain WCHECL1597 T were C16 : 0, C17 : 0cyclo and C18 : 1ω7c, which are in the quantitative range of other Enterobacter species while differentiated by the relatively higher amount (12.3 %) of iso-c16 : 1 I/C14 : 0 3-OH. The genomic DNA G+C content was 55.2 mol%. Strain WCHECL1597 T could be distinguished from all known Enterobacter species by its ability to ferment inositol but with a negative l-rhamnose and d-mannitol reaction. Genotypic and phenotypic characteristics from this study indicate that strain WCHECL1597 T represents a novel species of the genus Enterobacter, for which the name Enterobactersichuanensis sp. nov. is proposed. The type strain is WCHECL1597 T (=GDMCC1.1217 T =CCTCC AB 2017104 T =KCTC 52994 T ).