Open AccessPulveribacter suum gen. nov., sp. nov., isolated from a pig farm dust collector
Author(s) -
Jun Heo,
Ha-Young Cho,
In-Beom Heo,
Seungbum Hong,
Jeong-Seon Kim,
Soon-Wo Kwon,
Soojin Kim
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003082
Subject(s) - 16s ribosomal rna , biology , phylogenetic tree , strain (injury) , ribosomal rna , phosphatidylglycerol , microbiology and biotechnology , gene , bacteria , genomic dna , phylogenetics , botany , genetics , phospholipid , anatomy , membrane , phosphatidylcholine
An aerobic, Gram-stain-negative, polar-flagellated, rod-shaped bacterium, designated as SC2-7 T , was isolated from the dust collector at a pig farm located in Wanju-gun, Jeollabuk-do, Republic of Korea. Growth occurred at 10-37 °C (optimum, 28-30 °C), pH 6.0-10.0 (optimum, 7.0-8.0) and in the presence of 0-3 % (w/v) NaCl (optimum, 0 %) on Reasoner's 2A medium. The phylogenetic tree based on the 16S rRNA gene sequences revealed that strain SC2-7 T was a member of the family Comamonadaceae, forming a robust cluster with the genera Alicycliphilus, Oryzisolibacter and Melaminivora. The 16S rRNA gene sequences of strain SC2-7 T showed the highest sequence similarities to Alicycliphilus denitrificans K601 T (97.2 %), Oryzisolibacter propanilivorax EPL6 T (97.0 %), Melaminivora alkalimesophila CY1 T (96.9 %), Diaphorobacter polyhydroxybutyrativorans SL-205 T (96.6 %), Diaphorobacter nitroreducens NA10B T (96.6 %) and Melaminivora jejuensis KBB12 T (96.5 %). The tree based on the gyrA gene sequences also showed that strain SC2-7 T fell into a phylogenetic position similar to that based on the 16S rRNA gene sequences. The polar lipids present were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, an unidentified aminolipid and an unidentified phospholipid. The predominant quinone was ubiquione-8. The major fatty acids were summed feature 3 (including C16 : 1ω6c and/or C16 : 1ω7c), C16 : 0 and summed feature 8 (including C18 : 1ω6c and/or C18 : 1ω7c). The genomic DNA G+C content was 69.1 mol%. On the basis of the phenotypic, phylogenetic and chemotaxonomic data presented here, strain SC2-7 T represents a novel species of a new genus, for which the name Pulveribacter suum gen. nov., sp. nov., is proposed. The type strain of Pulveribacter suum is SC2-7 T (=KACC 19309 T =NBRC 113102 T ).