Open AccessFlavobacterium sediminis sp. nov., a starch-degrading bacterium isolated from tidal flat sediment
Author(s) -
Seung Seob Bae,
Mi Ree Kim,
Yoon-Hee Jung,
SungHyun Yang,
Kae Kyoung Kwon,
Kwang Hyun Baek
Publication year - 2018
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003081
Subject(s) - 16s ribosomal rna , biology , flavobacterium , strain (injury) , phylogenetic tree , bacteroidetes , bacteria , microbiology and biotechnology , flavobacteriaceae , genome size , ribosomal rna , gene , genome , pseudomonas , genetics , anatomy
A yellow-pigmented bacterium with the ability to degrade starch, designated MEBiC07310 T , was isolated from tidal flat sediment collected in Taean County, Republic of Korea. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain MEBiC07310 T was affiliated with the genus Flavobacterium in the phylum Bacteroidetes and showed that the strain was most closely related to Flavobacterium haoranii LQY-7 T (96.8 % similarity), followed by Flavobacterium indicum GPTSA 100-9 T (95.2 %) and Flavobacterium urocaniciphilum YIT 12746 T (94.6 %). Genome-based analysis of the average nucleotide identity (ANI) and in silico DNA-DNA hybridization (DDH) of strain MEBiC07310 T compared with F. haoranii LQY-7 T and F. indicum GPTSA 100-9 T yielded ANI values of 77.0 and 73.3 % and DDH values of 18.0±2.7 and 16.1±3.6 %, respectively. The DNA G+C content of strain MEBiC07310 T was 35.2 mol%. Cells of the strain were aerobic, Gram-stain-negative and rod-shaped, and negative for flexirubin-type pigments. Growth was observed at 17-43 °C (optimum 32 °C), at pH 5.0-8.0 (optimum pH 7.0) and with 0-3 % (w/v) NaCl (optimum 1 %). The major fatty acids (>10 %) of strain MEBiC07310 T were iso-C15 : 0, iso-C17 : 0 3-OH, summed feature 1 (iso-C15 : 1 H and/or C13 : 0 3-OH) and summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c). The major respiratory quinone was menaquinone MK-6. Based on its phenotypic and genotypic characteristics, strain MEBiC07310 T should be classified as representing a novel species of the genus Flavobacterium, for which the name Flavobacterium sediminis sp. nov. is proposed. The type strain is MEBiC07310 T (=KCTC 62132 T =JCM 32291 T ).