Open AccessDongshaea marina gen. nov., sp. nov., a facultatively anaerobic marine bacterium that ferments glucose with gas production
Author(s) -
Ssu Po Huang,
Tzu Yin Chen,
Jwo Sheng Chen,
Li Ting Wang,
Lina Huang,
S. T. Lin,
ChihLin Wei,
Shu-Yen Lin,
Pei Ling Wang,
Yi Min Chen,
Wung Yang Shieh
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003080
Subject(s) - biology , 16s ribosomal rna , gammaproteobacteria , phylogenetic tree , bacteria , phylogenetics , alphaproteobacteria , botany , microbiology and biotechnology , genus , biochemistry , gene , genetics
Two isolates of heterotrophic, facultatively anaerobic, marine bacteria, designated DM1 and DM2 T , were recovered from a lagoon sediment sample of Dongsha Island, Taiwan. Cells were Gram-reaction-negative rods. Nearly all of the cells were non-motile and non-flagellated during the late exponential to early stationary phase of growth, while a few of the cells exhibited motility with monotrichous flagellation. The two isolates required NaCl for growth and grew optimally at about 30 °C, 2-3 % NaCl and pH 7-8. They grew aerobically and could achieve anaerobic growth by fermenting d-glucose or other carbohydrates with production of acids and the gases, including CO 2 and H 2 . Ubiquinone Q-8 was the only respiratory quinone. Cellular fatty acids were predominated by C 16 : 0 , C 18 : 1 ω7 c and C 16 : 1 ω7 c . The major polar lipid was phosphatidylethanolamine. Strains DM1 and DM2 T had DNA G+C contents of 52.0 and 51.8 mol%, respectively, as determined by HPLC analysis. Phylogenetic analyses based on 16S rRNA gene sequences clearly indicated that the two isolates formed a distinct genus-level lineage in the family Aeromonadaceae of the class Gammaproteobacteria and was an outgroup with respect to a stable supragenic clade comprising species of the genera Oceanimonas , Oceanisphaera and Zobellella . The phylogenetic data and those from chemotaxonomic, physiological and morphological characterizations support the establishment of a novel species and genus inside the family Aeromonadaceae , for which the name Dongshaea marina gen. nov., sp. nov. is proposed. The type strain is DM2 T (=BCRC 81069 T =JCM 32096 T ).