Nocardioides silvaticus sp. nov., isolated from forest soil

A Gram-stain-positive, non-motile, rod-shaped bacterial strain S-34 T was isolated from forest soil. According to 16S rRNA gene sequence analysis, strain S-34 T was related to Nocardioides members and showed the highest similarities to Nocardioides thalensis NCCP-696 T (97.3 %) and Nocardioides panacisoliGsoil 346 T (97.0 %), Nocardioides litorisoli X-2 T (96.5 %) and Nocardioides immobilis FLL521 T (96.4 %). Phylogenetic trees showed that strain S-34 T fell within the cluster containing strain S-34 T and N. immobilis FLL521 T . The levels of DNA-DNA relatedness between strain S-34 T and N. thalensis CCTCC AB 2016296 T and between strain S-34 T and N. panacisoli KCTC 19470 T were 50.6 and 58.8 %, respectively. The genome orthoANI value between strain S-34 T and N. immobilis CCTCC AB 2017083 T was 82.4 %. Strain S-34 T had ll-diaminopimelic acid in the cell-wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol, four unknown phospholipids and one unknown lipid as the polar lipids, meanquinone-8(H4) as the only respiratory quinone and iso-C16 : 0, C17:1ω8c, C17:1ω6c, C17 : 0 and C17 : 0 10-methyl (tbsa) as the major fatty acids. The genome length of strain S-34 T was 4.53 Mb containing 52 contigs and with a DNA G+C content of 71.2 mol%. Strain S-34 T could be distinguished from the other Nocardioides members mainly based on the data of phylogenetic analyses, DNA-DNA hybridization, polar lipids and some biochemical differences. Therefore, strain S-34 T represents a novel species of the genus Nocardioides, for which the name Nocardioidessilvaticus sp. nov. is proposed. The type strain is S-34 T (=KCTC 49137 T =CCTCC AB 2018079 T ).