Open AccessClostridium composti sp. nov., a new anaerobic bacteria isolated from compost
Author(s) -
Yeseul Shin,
Jayoung Paek,
Ah Young Son,
Hongik Kim,
Joong-Ki Kook,
Woon Kee Paek,
Young-Hyo Chang
Publication year - 2018
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003074
Subject(s) - microbiology and biotechnology , clostridium , biology , clostridium butyricum , 16s ribosomal rna , bacteria , anaerobic bacteria , clostridiales , clostridiaceae , fermentation , biochemistry , toxin , genetics
Strain YH-tan31 T is a Gram-stain-positive, obligately anaerobic, spore-forming and mesophilic bacterium. The cells are rod-shaped and motile by means of peritrichous flagella. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate belongs to the genus Clostridium and is most closely related to Clostridium oryzae DSM 28571 T (97 % sequence identity), followed by Clostridium cellulovorans DSM 3052 T (92.4 %) and Clostridium butyricum KCTC 5387 T (92.1 %). The G+C content of the isolate was 30.9 mol%. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The predominant fatty acids were C14 : 0, C16 : 0, C16 : 0 DMA,and C19 : 0cis 11,12 DMA. The major end products of glucose fermentation were butyrate, acetate and lactate. This isolate represents a new species within the genus Clostridium, for which we propose the name Clostridiumcomposti sp. nov. (type strain, YH-tan31 T ; =KCTC 15630 T =JCM 32793 T ).