Open AccessParacoccus salipaludis sp. nov., isolated from saline–alkaline soil
Author(s) -
Xiaoyan Dong,
Guishan Zhang,
Qin Xiong,
Di Liu,
Dandan Wang,
Yunpeng Liu,
Gengwei Wu,
Li Pan,
Yu Luo,
Ruifu Zhang
Publication year - 2018
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003065
Subject(s) - biology , 16s ribosomal rna , strain (injury) , microbiology and biotechnology , phosphatidylglycerol , coccus (insect) , biochemistry , botany , gene , phosphatidylcholine , phospholipid , anatomy , membrane
A Gram-stain-negative, short ovoid- to coccus-shaped, aerobic, non-flagellated, and nonmotile strain, designated WN007 T , was isolated from the natural saline-alkali wetland soil. Growth occurred at 10-45 °C (optimum 33-37 °C), pH 6.5-10.0 (optimum, pH 7.5-8.0) and with 0-15 % (w/v) NaCl (optimum, 2-4 % NaCl). Catalase- and oxidase-positive. A comparison of the 16S rRNA gene sequence of WN007 T showed the highest sequence similarities to Paracoccus chinensis (97.5 %) and Paracoccus niistensis (97.4 %). The major respiratory quinone of strain WN007 T was Q10 and the fatty acid profile of strain WN007 T contained a predominant amount of summed feature 7 and small quantities of C10 : 0 3OH, C16 : 00 and C18 : 00. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, glycolipid, aminolipid and lipid. The genome revealed that the G+C content was 63.9 mol% and the DNA-DNA relatedness values between strain WN007 T and the type strains of P. chinensis CGMCC 1.7655 T and P. niistensis KCTC 22789 T were 46.9±2.3 and 42.4±1.7 %, respectively. This was also confirmed by the low average nucleotide identity values (<83.5 %) between strain WN007 T and the most closely related recognized Paracoccus species. According to these results, strain WN007 T represents a novel species of the genus Paracoccus, for which the name Paracoccussalipaludis sp. nov. is proposed. The type strain is WN007 T (=KCTC 52851 T =ACCC 19972 T ).