Open AccessZobellella endophytica sp. nov., isolated from the roots of Phragmites communis in the Kumtag Desert
Author(s) -
Zheng Shi,
Sun Qi-zhong,
Lisong Liang,
Xiaoxia Zhang,
Lubin Li,
Lei Liu
Publication year - 2018
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003064
Subject(s) - biology , 16s ribosomal rna , strain (injury) , phylogenetic tree , botany , genome size , genome , genus , bacteria , gene , genetics , anatomy
A Gram-negative, aerobic, non-spore-forming, rod-shaped, motile bacterium, named strain 59N8 T , was isolated from Phragmites communis roots in the Kumtag Desert. Phylogenetic analysis based on the 16S rRNA gene sequence showed that the isolate belongs to the genus Zobellella within the family Aeromonadaceae. The analysis showed that strain 59N8 T was most closely related to Zobellella taiwanensis ZT1 T . The average nucleotide identity value with Zobellella taiwanensis ZT1 T was 88.2 %, and the digital DNA-DNA hybridization value was 29.7±2.4 %, which was calculated using the Genome-to-Genome Distance Calculator. The G+C content of strain 59N8 T was 62.8 mol%. Strain 59N8 T grew at 0-5 % (w/v) NaCl (optimum, 0-4 %), pH 6.0-9.0 (optimum, 7.0-8.0) and at 10-45 °C. The major cellular fatty acids were summed feature 8 (C18 : 1ω7c/C18 : 1ω6c), summed feature 3 (C16 : 1ω7c/C16 : 1ω6c) and C16 : 0. The major polar lipids in strain 59N8 T were phosphatidylethanolamine and phosphatidylglycerol. Based on the chemotaxonomic, phylogenetic and phenotypic data, strain 59N8 T represents a novel species in the genus Zobellella, for which the name Zobellellaendophytica sp. nov. is proposed. The type strain is 59N8 T (=ACCC 60074 T =KCTC 62456 T ).