Open AccessA description of Mycobacterium chelonae subsp. gwanakae subsp. nov., a rapidly growing mycobacterium with a smooth colony phenotype due to glycopeptidolipids
Author(s) -
Byoung Jun Kim,
Bo Ram Kim,
Joseph Jeong,
Jung Hyun Lim,
Sang Hyuk Park,
Seung Heon Lee,
Chang Ki Kim,
Yoon Hoh Kook,
Bum Joon Kim
Publication year - 2018
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003056
Subject(s) - rpob , biology , mycobacterium chelonae , phylogenetic tree , housekeeping gene , mycobacterium , microbiology and biotechnology , 16s ribosomal rna , multilocus sequence typing , gene , genetics , genotype , bacteria , gene expression
Three rapidly growing mycobacterial strains, MOTTH4W, MOTT36W T and MOTT68W, were isolated from the sputa of three independent Korean patients co-infected with Mycobacterium yongonense Type II strains. The 16S rRNA gene sequences of all three strains were unique, which were closest to that of Mycobacterium chelonae subsp. bovis KCTC 39630 T (99.9 % similarity). Multilocus sequence typing analysis targeting 10 housekeeping genes including hsp65 and rpoB revealed the distinct phylogenetic location of these strains, which were clustered with M. chelonae subsp. chelonae ATCC 35752 T and M. chelonae subsp. bovis KCTC 39630 T . Phylogenetic analysis based on whole genome sequences revealed a 95.89 % average nucleotide identity (ANI) value with M. chelonae subsp. chelonae, slightly higher than the 95.0 % ANI criterion for determining a novel species. In addition, phenotypic characteristics such as a smooth colony morphology and growth inhibition at 37 °C, distinct MALDI-TOF MS profiles of extracted total lipids due to surface glycopeptidolipids, and distinct drug susceptibility profiles further supported the taxonomic characterization of these strains as representing a novel subspecies of Mycobacterium chelonae. Mycobacterium chelonae subsp. gwanakae subsp. nov. is proposed and the type strain is MOTT36W T (=KCTC 29127 T =JCM 32454 T ).