Open AccessPleomorphochaeta naphthae sp. nov., a new anaerobic fermentative bacterium isolated from an oil field
Author(s) -
Boussad Arroua,
Régis Grimaud,
Agnès Hirschler-Réa,
Patrick Bouriat,
Michel Magot,
Laurent Urios,
Anthony Ranchou-Peyruse
Publication year - 2018
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003048
Subject(s) - biology , 16s ribosomal rna , strain (injury) , fermentation , bacteria , phylogenetic tree , glycolipid , genus , biochemistry , gene , food science , botany , genetics , anatomy
A novel anaerobic fermentative bacterium, strain SEBR 4209 T , was isolated from a water sample of a Congolese oil field. Strain SEBR 4209 T is phylogenetically related to the genus Pleomorphochaeta, in the family Spirochaetaceae. Its closest relatives are Pleomorphochaeta caudata SEBR 4223 T (94.5 % 16S rRNA gene sequence similarity) and Pleomorphochaeta multiformis MO-SPC2 T (94.3 % similarity). Like the other members of this genus, cells have a pleomorphic morphology, in particular an annular shape and long stalks. Optimal growth was observed at 37 °C, at pH between 6.8 and 7.0, and with 40 g l -1 NaCl. This strain was only able to grow by fermentation of carbohydrates. The fermentation products from glucose utilization were acetate, ethanol, CO2 and H2. Predominant fatty acids were C14 : 0, C14 : 0 DMA, C16 : 0 and C16 : 1ω7c. The major polar lipids were phosphoglycolipids, phospholipids and glycolipids. The G+C content of the DNA was 29.6 mol%. Based on phenotypic characteristics and phylogenetic traits, strain SEBR 4209 T is considered to represent a novel species of the genus Pleomorphochaeta, for which the name Pleomorphochaetanaphthae sp. nov. is proposed. The type strain is SEBR 4209 T (=DSM 104684 T =JCM 31871 T ).