Open AccessStreptomyces durbertensis sp. nov., isolated from saline–alkali soil
Author(s) -
Yu Yang,
Yansong Fu,
Xiaowei Guo,
Rui Yan,
Han Wang,
Junwei Zhao,
Xiangjing Wang,
Ji Zhang,
Wensheng Xiang
Publication year - 2018
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003047
Subject(s) - biology , 16s ribosomal rna , rpob , actinobacteria , streptomyces , phylogenetic tree , strain (injury) , housekeeping gene , clade , botany , diamino acid , actinomycetales , microbiology and biotechnology , gene , bacteria , genetics , gene sequence , anatomy , gene expression
A novel strain of actinobacteria, designated NEAU-S1GS20 T , was isolated from a saline-alkali soil collected from Heilongjiang Province, north-east China, and characterized using a polyphasic approach. Strain NEAU-S1GS20 T exhibited morphological, cultural and chemotaxonomic features consistent with its classification as representing a member of the genus Streptomyces. Growth occurred at 18‒45 °C, at pH 6.0‒10.0 and in the presence of 10 % (w/v) NaCl. Whole-cell hydrolysates mainly contained glucose and ribose. The predominant menaquinones were MK-9(H4) and MK-9(H6). The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and two unidentied phospholipids. The major cellular fatty acids (>10 %) were iso-C16 : 0, iso-C17 : 0, anteiso-C17 : 0 and C16 : 0. The G+C content of the DNA was 72.8 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain NEAU-S1GS20 T formed a distinct clade within the genus Streptomyces and was closely related to Streptomyces xinghaiensis CCTCC AA 208049 T (98.4 % similarity), Streptomyces chumphonensis JCM 18522 T (98.1 %) and Streptomyces palmae JCM 31289 T (98.1 %). Multilocus sequence analysis (MLSA) using five housekeeping genes (atpD, gyrB, recA, rpoB and trpB) showed that the MLSA distance of strain NEAU-S1GS20 T to the most closely related species was greater than the 0.007 threshold. A combination of DNA-DNA hybridization results and differences in certain phenotypic characteristics demonstrated that strain NEAU-S1GS20 T could be distinguished from its closest phylogenetic relatives. Therefore, strain NEAU-S1GS20 T represents a novel species of the genus Streptomyces, for which the name Streptomyces durbertensis sp. nov. is proposed. The type strain is NEAU-S1GS20 T (=CCTCC AA 2017006 T =DSM 104538 T ).