Open AccessPaenibacillus paeoniae sp. nov., a novel endophytic bacterium isolated from leaf of Paeonia lactiflora Pall.
Author(s) -
Xiao-Rui Yan,
Tuo Li
Publication year - 2018
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003042
Subject(s) - biology , 16s ribosomal rna , paeonia lactiflora , botany , bacteria , microbiology and biotechnology , phylogenetic tree , phosphatidylglycerol , paenibacillus , strain (injury) , chemotaxonomy , taxonomy (biology) , gene , biochemistry , phospholipid , medicine , genetics , alternative medicine , pathology , anatomy , membrane , phosphatidylcholine
A Gram-stain-positive, aerobic, rod-shaped, endospore-forming bacterium, designated strain M4BSY-1 T , was originally isolated from a surface-sterilized leaf of Paeonia lactiflora Pall. in Guizhou, China. The bacterium was characterized by a polyphasic approach to determine its taxonomic position. 16S rRNA gene sequence comparison revealed that strain M4BSY-1 T belongs to the genus Paenibacillus and most closely to Paenibacilluspinioli NB5 T (98.31 % similarity). Neither substrate nor aerial mycelia formed, and no diffusible pigments were observed on the media tested. Strain M4BSY-1 T grew in the pH range 7.0-13.0 (optimum, 7.0-8.0), at temperatures between 10-37 °C (optimum, 30 °C) and at 0-5 % (w/v) NaCl (optimum, 1-2 %). The predominant menaquinone was MK-7. The major fatty acids were anteiso-C15 : 0, C16 : 0 and iso-C16 : 0. The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified unknown aminophospholipids and three unidentified phospholipids. The DNA G+C content was 48.8 mol%. According to the phylogenetic, phenotypic and chemotaxonomic evidence, strain M4BSY-1 T was clearly distinguishable from other species with validly published names in the genus Paenibacillus and should therefore be classified as a novel species, and we suggest the name Paenibacillus paeoniae sp. nov. The type strain is M4BSY-1 T (=KCTC 33997 T =CGMCC 1.13667 T ).