Open AccessDesertimonas flava gen. nov., sp. nov. isolated from a desert soil, and proposal of Ilumatobacteraceae fam. nov.
Author(s) -
Mipeshwaree Devi Asem,
Lei Shi,
JianYu Jiao,
Dong Wang,
Ming-Xian Han,
Lei Dong,
Feng Liu,
Nimaichand Salam,
WenJun Li
Publication year - 2018
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003038
Subject(s) - biology , 16s ribosomal rna , phylogenetic tree , taxon , diamino acid , clade , taxonomy (biology) , diaminopimelic acid , phylogenetics , microbiology and biotechnology , strain (injury) , bacteria , genus , desert climate , botany , gene , genetics , gene sequence , ecology , anatomy , arid
A non-motile, coccobacilli-shaped and yellow-coloured bacterium, designated strain SYSU D60003 T , was isolated from a desert soil sample. Cells were Gram-stain-positive, catalase-negative and oxidase-positive. The whole cell hydrolysates contained ll-diaminopimelic acid as the diagnostic amino acid. The major fatty acids were C16 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and iso-C16 : 0. The respiratory menaquinones were MK-9(H8), MK-9(H4) and MK-9(H6). The DNA G+C content was determined to be 70.2 % (genome). The polar lipids detected were diphosphatidylglycerol, an unidentified glycolipid and seven unidentified polar lipids. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SYSU D60003 T belonged to the order Acidimicrobiales (class Acidimicrobiia), but formed a clade closely linked to members of the genus Ilumatobacter. Data from a polyphasic taxonomy study suggested that the isolate represents a novel species of a novel genus in the order Acidimicrobiales, for which the name Desertimonas flava gen. nov., sp. nov. is proposed. The type strain of the proposed new taxon is SYSU D60003 T (=KCTC 39917 T =NBRC 112924 T ). Additionally, the new taxon along with the genus Ilumatobater (family unassigned) were distinctly separated from the related families Acidimicrobiaceae, Iamiaceae and 'Microtrichaceae' in the phylogenetic trees, besides presenting a unique 16S rRNA gene signature nucleotides. Therefore, we propose a new family Ilumatobacteraceae fam. nov. within the order Acidimicrobiales to accommodate members of these two genera.