Open AccessMarinifilum breve sp. nov., a marine bacterium isolated from the Yongle Blue Hole in the South China Sea and emended description of the genus Marinifilum
Author(s) -
Tianyu Fu,
Jia Cao,
Liang Fu,
Shun Zhou,
Peng Yao,
Rui Du,
Hao Sun,
Zuosheng Yang,
Xiaochong Shi,
Xiaohua Zhang
Publication year - 2018
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003027
Subject(s) - biology , 16s ribosomal rna , strain (injury) , bacteria , genus , glycolipid , microbiology and biotechnology , botany , genetics , anatomy , biochemistry
A Gram-stain-negative, facultative anaerobic, non-motile, short-clavate and non-flagellated marine bacterium strain, designated JC075 T , was isolated from the Yongle Blue Hole in the South China Sea. Based on the 16S rRNA gene sequence, strain JC075 T was found to be closely related to Marinifilum albidiflavum FB208 T (97.10 %), Marinifilum flexuosum DSM 21950 T (96.43 %) and Marinifilum fragile JCM 15579 T (95.58 %), with less than 90.24 % sequence similarity to other genera of the family Marinifilaceae. The growth temperature was in the range of 10-37 °C, and the optimum temperature was 16 °C. Optimal growth occurred at pH 7.0 and in the presence of 3 % (w/v) NaCl. The isoprenoid quinone of strain JC075 T was identified as menaquinone-7 and the predominant fatty acids (>10 %) were iso-C15 : 0 (47.9 %), summed feature 9 (C17 : 1 or/and iso-C17 : 1ω9c; 18.7 %) and iso-C17 : 0 3-OH (14.9 %). The major polar lipids were one phosphatidylethanolamine, one phospholipid, one aminophospholipid, one glycolipid, one aminolipid and two unidentified lipids. The DNA G+C content of strain JC075 T was 35.8 mol%. On the basis of polyphasic analysis, strain JC075 T was considered to represent a novel species of the genus Marinifilum, for which the name Marinifilumbreve sp. nov. is proposed. The type strain is JC075 T (=KCTC 15646 T =MCCC 1K03477 T =JCM 32401 T ).