Open AccessMarinobacter bohaiensis sp. nov., a moderate halophile isolated from benthic sediment of the Bohai Sea
Author(s) -
Shanshan Xu,
Dandan Wang,
Yuxi Wei,
Qiu Cui,
Wenli Li
Publication year - 2018
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003025
Subject(s) - biology , halophile , benthic zone , sediment , oceanography , ecology , bacteria , paleontology , geology
A Gram-stain-negative, motile, aerobic and rod-shaped bacterial strain, designated T17 T , was isolated from benthic sediment sampled at Jiaozhou Bay, Bohai Sea, China, and its taxonomic position was investigated. The 16S rRNA gene sequence of strain T17 T exhibited the highest similarity values to those of the type strain Marinobacter lacisalsi FP2.5 (96.2 %) and Marinobacter koreensis DD-M3 T (96.2 %). Strain T17 T grew optimally at 35 °C, pH 7.0-8.0 and in the presence of 6.0-10.0 % (w/v) NaCl. The predominant ubiquinone in strain T17 T was identified as Q-9. The major fatty acids of strain T17 T were C12 : 0, C16 : 0 and C16 : 0 10-CH3. The major polar lipids of strain T17 T were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidglycerol, an unidentified aminolipid and an unidentified phospholipid. The DNA G+C content of strain T17 T was 63.0 mol%. The draft genome sequence of strain T17 T includes 4 755 891 bp in total (N50=2 856 325 bp) with a medium read coverage of 100.0x and 11 scaffolds. In silico DNA-DNA hybridization with the three type strains showed 20.3, 19.7 and 19.9 % relatedness to Marinobacter santoriniensis NKSG1 T , Marinobacter segnicrescens SS11B1-4 T and Marinobacter daqiaonensis CGMCC 1.9167 T , respectively. On the basis of the phenotypic, phylogenetic, genomic and chemotaxonomic properties, strain T17 T is considered to represent a novel species within the genus Marinobacter, for which the name Marinobacterbohaiensis sp. nov. is proposed. The type strain is T17 T (=KCTC 52710 T =MCCC 1K03282 T ).