Open AccessRubrobacter indicoceani sp. nov., a new marine actinobacterium isolated from Indian Ocean sediment
Author(s) -
Rou-Wen Chen,
Kexin Wang,
FaZuo Wang,
Yuan-Qiu He,
Lijuan Long,
Xinpeng Tian
Publication year - 2018
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003018
Subject(s) - biology , 16s ribosomal rna , phylogenetic tree , strain (injury) , peptidoglycan , botany , genus , microbiology and biotechnology , genome size , gene , genome , genetics , anatomy
A novel mesophilic marine actinobacterial strain, designated as SCSIO 08198 T , was isolated from a deep-sea sediment sample collected from the Indian Ocean. The strain was Gram-stain-positive, rod-shaped and salmon pink in colour. Good growth occurred on marine agar with 1-5 % (w/v) NaCl and incubation at 28 °C for more than a fortnight. Sensitive to short ultraviolet radiation. Analysis of 16S rRNA gene sequences revealed that strain SCSIO 08198 T had the highest similarity of 97.2 % to Rubrobacter radiotolerans DSM 5868 T , and loosely related (<94.2 %) to all other species in the genus Rubrobacter. Phylogenetic analysis based on nearly complete 16S rRNA gene sequences revealed that the novel isolate shared a lineage with members of the genus Rubrobacter. The total cellular fatty acid profile was dominated by C16 : 0 12-methyl. MK-8 was the main menaquinone. The peptidoglycan type was A3α (l-Lys-l-Ala). The major phospholipids were diphosphatidylglycerol, phosphatidylglycerol and unidentified phospholipids. Based on the whole genome sequence analysis, the genome size is 3 078 689 bp with DNA G+C value of 63.8 mol%, including one circular chromosome and two plasmids. Based on these polyphasic data, a new species, Rubrobacterindicoceani sp. nov., is proposed, with the type strain SCSIO 08198 T (=DSM 105148 T =CGMCC 1.16398 T ).