Open AccessPedobacter chitinilyticus sp. nov., a chitin-degrading bacterium isolated from wheat leaf tissue
Author(s) -
Lingli Zhang,
Lian Gan,
Zhibin Xu,
Feng Yang,
Yan Li,
Xiangtao Fan,
Xiaofeng Liu,
Yongqiang Tian,
Yumei Dai
Publication year - 2018
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003017
Subject(s) - biology , 16s ribosomal rna , strain (injury) , phylogenetic tree , chitin , genomic dna , bacteria , spore , botany , microbiology and biotechnology , genus , dna , gene , genetics , anatomy , biochemistry , chitosan
A bacterium, designated strain CM134L-2 T , was isolated from a chitin-enriched wheat leaf microbiome in Chengdu, Sichuan province, China. It was Gram-stain-negative, strictly aerobic, non-spore-forming, motile, rod-shaped, and bright yellow in colour. Strain CM134L-2 T grew at 4-35 °C, at pH 6.0-9.0 and could use chitin as the only carbon resource. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CM134L-2 T was most closely related to Pedobacter nanyangensis Q-4 T (97.7 %) and Pedobacter zeaxanthinifaciens TDMA-5 T (97.4 %). Digital DNA-DNA hybridization (dDDH) values between strain CM134L-2 T with these two type strains were 26.8 and 20.8 %, respectively, and average nucleotide identity (ANI) values were 83.2 and 76.2 %; these values are lower than the proposed and generally accepted species boundaries of 70 % for dDDH and 95-96 % for ANI, which suggests strain CM134L-2 T represents a novel species. The genomic DNA G+C content of strain CM134L-2 T was 39.3 mol%, menaquinone-7 was the major respiratory quinone, phosphatidylethanolamine was the major polar lipid and the major components of the cellular fatty acids were iso-C15 : 0, and C16 : 1ω7c/C16 : 1ω6c (summed feature 3); these features supported the affiliation of strain CM134L-2 T to the genus Pedobacter. Overall, strain CM134L-2 T belongs to the genus Pedobacter, but can be classified as a novel species, for which the name Pedobacter chitinilyticus sp. nov. is proposed. The type strain is CM134L-2 T (=CGMCC 1.16520 T =KCTC 62643 T ).