Open AccessChitinophaga parva sp. nov., a new member of the family Chitinophagaceae, isolated from soil in a chemical factory
Author(s) -
Zhuang Ke,
Mu-Ji Yang,
Weibin Jia,
Yang Mu,
Jianlong Li,
Dian Chen,
Kai Chen,
Jiandong Jiang
Publication year - 2018
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003006
Subject(s) - biology , 16s ribosomal rna , phylogenetic tree , strain (injury) , genomic dna , microbiology and biotechnology , bacteria , phylogenetics , glycolipid , gene , botany , genetics , biochemistry , anatomy
A Gram-stain-negative bacterium, designated LY-1 T , was isolated from the soil sample collected from a chemical factory in Fuyang city, Anhui province, China. Cells of strain LY-1 T were strictly aerobic, non-motile and rod-shaped. Strain LY-1 T grew optimally at pH 7.0 and at 30-35 °C. The taxonomic position was investigated using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain LY-1 T was a member of the genus Chitinophaga and showed the highest sequence similarity to Chitinophaga costaii A37T2 T (97.5 %) and lower (<97.0 %) sequence similarity to other known Chitinophaga species. Chemotaxonomic analysis revealed that strain LY-1 T possessed menaquinone-7 as the major isoprenoid quinone; and iso-C15 : 0 (46.4 %), C16 : 1ω5c (27.8 %) and iso-C17 : 0 3-OH (9.0 %) were the predominant fatty acids. The polar lipids of strain LY-1 T consisted of phosphatidylethanolamine, three unidentified phosphoaminolipids, one unidentified phospholipid, four unidentified lipids, two unidentified aminolipids and two unidentified glycolipids. The genomic DNA G+C content of strain LY-1 T was 52.4 mol% based on total genome calculations. The average nucleotide identity and the digital DNA-DNA hybridization value of the draft genomes between strain LY-1 T and strain A37T2 T were 76.8 and 19.8 %, respectively. Based on the phylogenetic and phenotypic characteristics, chemotaxonomic data, and DNA-DNA hybridization, strain LY-1 T is considered a novel species of the genus Chitinophaga, for which the name Chitinophagaparva sp. nov. (type strain LY-1 T =CCTCC AB 2018018 T =KCTC 62444 T ) is proposed.