Open AccessGeobacillus yumthangensis sp. nov., a thermophilic bacterium isolated from a north-east Indian hot spring
Author(s) -
Ishfaq Nabi Najar,
Mingma Thundu Sherpa,
Sayak Das,
Kamalesh Verma,
Vikash Kumar Dubey,
Nagendra Thakur
Publication year - 2018
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003002
Subject(s) - thermophile , biology , 16s ribosomal rna , strain (injury) , hot spring , phylogenetic tree , bacteria , spore , genomic dna , microbiology and biotechnology , botany , gene , biochemistry , genetics , paleontology , anatomy
A thermophilic, spore-forming, rod-shaped bacterium isolated from the Yumthang hot spring in North Sikkim, India was subjected to taxonomic studies. The thermophilic bacterial isolate was designated as strain AYN2 T . Cells were Gram-stain-positive, aerobic, motile, rod-shaped, catalase-positive and methyl red-negative. Strain AYN2 T was able to grow in the pH range from 6 to 10 (optimum, pH 7.5-8.0), at 40-70 °C (60 °C) and in NaCl concentrations of 0-4 % (1 %). The major cellular fatty acids were iso-C15 : 0 (12.8 %), iso-C16 : 0 (13.9 %) and iso-C17 : 0 (13.8 %). No matches were found in the rtsba6 Sherlock libraries. The G+C content of the genomic DNA was 42.11 mol%. Based on phylogenetic analysis of the 16S rRNA gene sequences, strain AYN T showed highest sequence similarity to the type strain of Geobacillus toebii (96 %). However, the phenotypic properties of strain AYN2 T were clearly distinct from those of G. toebii and related species. On the basis of polyphasic analysis, strain AYN2 T represents a novel species in the genus Geobacillus, for which the name Geobacillus yumthangensis sp. nov. is proposed. The type strain is AYN2 T (MTCC=12749=KCTC=33950= JCM 32596).