Open AccessPichia nanzhaoensis sp. nov. and Pichia paraexigua f.a., sp. nov., two yeast species isolated from rotting wood
Author(s) -
Wanli Gao,
Kai-Fang Liu,
Lunguang Yao,
Feng-Li Hui
Publication year - 2018
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.002989
Subject(s) - biology , pichia , yeast , internal transcribed spacer , ascus (bryozoa) , botany , strain (injury) , microbiology and biotechnology , ribosomal rna , pichia pastoris , gene , spore , genetics , ascospore , recombinant dna , anatomy
Four yeast strains were isolated from rotting wood samples collected in the Baotianman Nature Reserve in Henan Province, Central China. On the basis of sequence analysis of the D1/D2 domains of the large subunit rRNA gene and the internal transcribed spacer regions, they were suggested to be two novel species of the genus Pichia. Pichia nanzhaoensis sp. nov. produces one to four spherical ascospores per ascus, and is most closely related to Candida pseudolambica. Pichia paraexigua f.a., sp. nov. is a sister taxa to Pichia exigua, but the formation of ascospores was not observed on various sporulation media. P. nanzhaoensis sp. nov. can weakly assimilate inulin, whereas P. paraexigua sp. nov. can weakly assimilate d-glucosamine. The type strain of Pichia nanzhaoensis is NYNU 178136 T (=CICC 33279 T =CBS 15346 T ) and the type strain of Pichia paraexigua is NYNU 178135 T (=CICC 33278 T =CBS 15237 T ).