Open AccessSphingobacterium haloxyli sp. nov., an endophytic bacterium isolated from Haloxylon ammodendron stems in Kumtag desert
Author(s) -
Lei Liu,
Nan Hui,
Lisong Liang,
Xiaoxia Zhang,
Lubin Li,
Sun Qi-zhong
Publication year - 2018
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.002982
Subject(s) - biology , 16s ribosomal rna , strain (injury) , phylogenetic tree , bacteria , botany , microbiology and biotechnology , gene , genetics , anatomy
A Gram-stain-negative, non-motile, aerobic, non-spore-forming, rod-shaped, bacterial strain, designated 5JN-11 T , was isolated from Haloxylonammodendron stems in Kumtag desert, Xinjiang province, China. Strain 5JN-11 T grew at salinities of 0-6 % (w/v; optimum 0-2 %), a pH of 7.0-9.0 (pH 7.0-8.0) and temperatures of 20-42 °C (28-30 °C). Based on 16S rRNA gene sequences, the strain was designated a member of the genus Sphingobacterium and the phylogenetic analysis showed that strain 5JN-11 T shared the highest similarity to Sphingobacterium gobiense H7 T , followed by Sphingobacterium chuzhouense DH-5 T and Sphingobacterium arenae H-12 T . The unfinished draft genome of strain 5JN-11 T was 4.69 Mb. The G+C content of strain 5JN-11 T was 42.8 mol%. The average nucleotide identity to S. gobiense H7 T was 90.5 %. The respiratory quinone was MK-7, and the major polar lipids were phosphatidylethanolamine and phosphoglycolipid. The predominant cellular fatty acids were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and iso-C17 : 0 3-OH. On the basis of phenotypic, genotypic and phylogenetic evidence, strain 5JN-11 T represents a novel species in the genus Sphingobacterium, for which the name Sphingobacteriumhaloxyli sp. nov. is proposed. The type strain is 5JN-11 T (=ACCC 60072 T =KCTC 62457 T ).