Open AccessListeria goaensis sp. nov.
Author(s) -
Swapnil Doijad,
Krupali V. Poharkar,
Satyajit B. Kale,
Savita Kerkar,
D. R. Kalorey,
N. V. Kurkure,
Deepak B. Rawool,
S.V.S. Malik,
Rafed Yassin Ahmad,
Martina Hudel,
S. P. Chaudhari,
Birte Abt,
Jörg Overmann,
Markus Weigel,
Torsten Hain,
Sukhadeo B. Barbuddhe,
Trinad Chakraborty
Publication year - 2018
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.002980
Subject(s) - biology , listeria , 16s ribosomal rna , microbiology and biotechnology , ribosomal rna , bacteria , listeria monocytogenes , genetics , gene
Two Listeria-like isolates obtained from mangrove swamps in Goa, India were characterized using polyphasic combinations of phenotypic, chemotaxonomic and whole-genome sequence (WGS)-based approaches. The isolates presented as short, non-spore-forming, Gram-positive rods, that were non-motile, oxidase-negative, catalase-positive and exhibited α-haemolysis on 5 % sheep- and horse-blood agar plates. The 16S rRNA gene sequences exhibited 93.7-99.7 % nucleotide identity to other Listeria species and had less than 92 % nucleotide identity to species of closely related genera, indicating that the isolates are de facto members of the genus Listeria. Their overall fatty acid composition resembled that of other Listeria species, with quantitative differences in iso C15 : 0, anteiso C15 : 0, iso C16 : 0, C16 : 0, iso C17 : 0 and anteiso C17 : 0 fatty acid profiles. Phylogeny based on 406 core coding DNA sequences grouped these two isolates in a monophyletic clade within the genus Listeria. WGS-based average nucleotide identity and in silico DNA-DNA hybridization values were lower than the recommended cut-off values of 95 and 70 %, respectively, to the other Listeria species, indicating that they are founding members of a novel Listeria species. We suggest the name Listeriagoaensis sp. nov. be created and the type strain is ILCC801 T (=KCTC 33909;=DSM 29886;=MCC 3285).