Open AccessKomagataeibacter cocois sp. nov., a novel cellulose-producing strain isolated from coconut milk
Author(s) -
Longxiang Liu,
Sixin Liu,
Yanmei Wang,
Jicai Bi,
Chen Huamei,
Jun Deng,
Cui Zhang,
Qisong Hu,
Congfa Li
Publication year - 2018
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.002947
Subject(s) - biology , 16s ribosomal rna , acetic acid bacteria , strain (injury) , mannitol , phylogenetic tree , rpob , galactose , biochemistry , microbiology and biotechnology , acetic acid , gene , anatomy
Phylogenetic analysis was performed on a cellulose-producing strain, designated WE7 T , isolated from contaminated coconut milk. The analysis utilized nearly complete 16S rRNA gene sequences, as well as concatenated partial sequences of the housekeeping genes dnaK, groEL and rpoB, and allowed identification of the strain as belonging to the genus Komagataeibacter. DNA-DNA correlation or average nucleotide identity analysis was performed between WE7 T and its closest phylogenetic neighbours, and the resulting values were below the species level (<70 % and <95 %), suggesting that the strain represents a novel species in genus Komagataeibacter. Strain WE7 T was coupled with Komagataeibacter species more tightly than with Gluconacetobacter species in a 16S rRNA gene sequence phylogenetic tree. Strain WE7 T can be differentiated from closely related Komagataeibacter and Gluconacetobacter entanii species by the ability to grow on the carbon sources d-mannitol, sodium d-gluconate and glycerol, the ability to form acid by d-fructose, sucrose, d-mannitol, d-galactose and ethanol, and the ability to grow without acetic acid. The major fatty acid of WE7 T is C18 : 1ω9c (52.3 %). The DNA G+C content of WE7 T is 63.2 mol%. The name Komagataeibacter cocois sp. nov. is proposed, with the type strain WE7 T (=CGMCC 1.15338 T =JCM 31140 T ).