Open AccessEmcibacter congregatus sp. nov., isolated from sediment cultured in situ
Author(s) -
Zhe Zhao,
Xia Shen,
Chen Wu,
Xiaoyun Yu,
GeYi Fu,
Cong Sun,
Min Wu
Publication year - 2018
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.002906
Subject(s) - 16s ribosomal rna , biology , phylogenetic tree , strain (injury) , phosphatidylglycerol , genomic dna , bacteria , genus , bacterial strain , microbiology and biotechnology , gene , botany , genetics , phospholipid , anatomy , membrane , phosphatidylcholine
A Gram-negative, aerobic, motile, rod-shaped, pale-yellow bacterial strain, designated as ZYL T , isolated from a cultured in situ sediment sample collected from the East China Sea coast, was studied using a polyphasic taxonomic approach. Strain ZYL T grew at 4-30 °C (optimum, 25 °C), at pH 6.0-8.5 (pH 7.0) and with 0-7.0 % (w/v) NaCl (2.0 %). Results of phylogenetic analysis based on 16S rRNA gene sequences clearly showed that strain ZYL T and Emcibacter nanhaiensis HTCJW17 T , which was most closely related to strain ZYL T with 93.6 % sequence similarity, clustered together. The genomic DNA G+C content was 51.5 % (genome sequence). The quinone system was composed only of ubiquinone-10. Strain ZYL T possessed C18 : 1ω7c and/or C18 : 1ω6c (summed feature 8), iso-C15 : 0 2-OH and/or C16 : 1ω7c (summed feature 3), C14 : 0 2-OH and C14 : 0 as the major fatty acids. The content of summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1ω7c) in strain ZYL T was far greater than that in E. nanhaiensis. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, three unidentified aminophospholipids, three unidentified phospholipids, one unidentified aminolipid and four unidentified lipids. One unidentified aminophospholipid and two unidentified lipids present in strain ZYL T were not found in E. nanhaiensis in this research. On the basis of phenotypic, phylogenetic and chemotaxonomic data, strain ZYL T (=KCTC 62328 T =JCM 32378 T =MCCC 1K03526 T ) represents a novel species of the genus Emcibacter for which the name Emcibacter congregatus sp. nov. is proposed.