Open AccessNesterenkonia endophytica sp. nov., isolated from roots of Glycyrrhiza uralensis
Author(s) -
Li Li,
Yanqiong Li,
Yu-Song Fu,
Hui Zhang,
Dalal Hussien M. Alkhalifah,
Nimaichand Salam,
Wael N. Hozzein,
Mipeshwaree Devi Asem,
Wenjun Li
Publication year - 2018
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.002905
Subject(s) - biology , 16s ribosomal rna , phylogenetic tree , strain (injury) , phylogenetics , ribosomal rna , sequence analysis , botany , gene , microbiology and biotechnology , genetics , anatomy
A Gram-positive and non-motile actinobacterium, designated strain EGI 60016 T , was isolated from healthy roots of Glycyrrhiza uralensis F. collected from Xinyuan County, Xinjiang Province, China. The 16S rRNA gene sequence of strain EGI 60016 T was found to show 97.5 and 97.3 % sequence similarities to Nesterenkonia rhizosphaerae EGI 80099 T and Nesternkonia massiliensis NP1 T , respectively. The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain EGI 60016 T formed a distinct clade with N. rhizosphaerae EGI 80099 T and N. massiliensis NP1 T . The polar lipids detected for strain EGI 60016 T were diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, phosphatidylinositol, an unidentified glycolipid, an unidentified lipid and an unidentified phospholipid. The DNA G+C content was determined to be 64.1 mol%. Other chemotaxonomic features of strain EGI 60016 T included MK-7, MK-8 and MK-9 as the respiratory quinones, and anteiso-C15 : 0 and anteiso-C17 : 0 as the major fatty acids. Based on the results of the phylogenetic analysis supported by morphological, physiological, chemotaxonomic and other differentiating phenotypic characteristics, strain EGI 60016 T is considered to represent a novel species of the genus Nesterenkonia, for which the name Nesterenkonia endophytica sp. nov. is proposed. The type strain is EGI 60016 T (=CCTCC AB 2017176 T =NBRC 112398 T ).