Open AccessPhreatobacter cathodiphilus sp. nov., isolated from a cathode of a microbial fuel cell
Author(s) -
Soojin Kim,
Jae-Hyung Ahn,
Jun Heo,
Hayoung Cho,
Hang-Yeon Weon,
SeungBeom Hong,
Jeong-Seon Kim,
Soon-Wo Kwon
Publication year - 2018
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.002904
Subject(s) - 16s ribosomal rna , biology , phosphatidylglycerol , strain (injury) , microbiology and biotechnology , flagellum , bacteria , gene , biochemistry , phosphatidylcholine , phospholipid , genetics , anatomy , membrane
A novel bacterial strain, S-12 T , of a member of the genus Phreatobacterwas isolated from a cathode of a microbial fuel cell from Suwon City, South Korea. Cells were Gram-staining-negative, aerobic, non-sporulating rods, motile by means of a polar flagellum, and formed white round colonies. The strain grew at the range of 10-40 °C (optimum, 28-30 °C), pH 6.0-10.0 (optimum 7.0-8.0) and 0-1 % NaCl. The 16S rRNA gene sequence analysis showed the relatedness of S-12 T to Phreatobacter stygiusYC6-17 T (98.2 %) and Phreatobacter oligotrophusPI_21 T (98.1 %). The major respiratory quinone was ubiquinone Q-10. Polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine and an unidentified lipid. The major fatty acids were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c). The DNA G+C content was 69.3 mol%. On the basis of its differences from species of the genus Phreatobacter with validly published names, strain S-12 T is identified as representing a novel species, for which the proposed name is Phreatobactercathodiphilus sp. nov., with S-12 T as the type strain (=KACC 18497 T =JCM 31612 T ).