Solitalea longa sp. nov., isolated from freshwater and emended description of the genus Solitalea

A Gram-negative, strictly aerobic, oxidase-positive, catalase-negative and yellow-pigmented bacterium, designated strain HR-AV T , was isolated from a water sample of the Han River. Cells were elongated rods with gliding motility without flagellum. Growth was observed at 5-30 °C (optimum, 20 °C), pH 7-8 and 0-0.5 % NaCl. The major respiratory quinone was menaquinone-7. The major fatty acids were iso-C15 : 0, summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c) and anteiso-C15 : 0. The polar lipids comprised phosphatidylethanolamine, an unidentified amino lipid and five unidentified lipids. The DNA G+C content of strain HR-AV T was 38.4 mol%. Results of phylogenetic analysis based on 16S rRNA gene sequences revealed that strain HR-AV T belonged to the family Sphingobacteriaceae in the phylum Bacteroidetes and formed a phylogenic lineage with Solitalea canadensis DSM 3403 T and Solitalea koreensis R2A36-4 T . Strain HR-AV T was most closely related to S. canadensis DSM 3403 T and S. koreensis R2A36-4 T with 97.3 and 94.0 % 16S rRNA gene sequence similarities, respectively, and then had low similarities (below 90.9 %) with other bacteria with validly published names. Average nucleotide identity and in silico DNA-DNA hybridization values between strain HR-AV T and S. canadensis were 74.0 and 19.7 %, respectively. Based on these results, strain HR-AV T represents a novel species of the genus Solitalea, for which the name Solitalea longa sp. nov. is proposed. The type strain is HR-AV T (=KACC 19411 T =JCM 32259 T ). An emended description of the genus Solitalea is also proposed.