Open AccessRuegeria kandeliae sp. nov., isolated from the rhizosphere soil of a mangrove plant Kandelia candel
Author(s) -
Lv Zhang,
Kai-Ling Wang,
Qi Yin,
Jinyou Liang,
Ying Xü
Publication year - 2018
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.002894
Subject(s) - kandelia candel , biology , rhizosphere , mangrove , botany , ecology , bacteria , genetics
A Gram-negative, rod-shaped and motile bacterium, designated strain J95 T , was isolated from the rhizosphere soil of a mangrove plant Kandeliacandel (L.) Druce in Mai Po Nature Reserve, Hong Kong. Growth of strain J95 T was observed at pH 5.0-8.5 (optimum, 6.0-7.0), between 10-40 °C (30-37 °C) and in the presence of 0-9 % (w/v) NaCl (0.5-3 %). Chemotaxonomic analysis showed ubiquinone-10 as the predominant respiratory quinone and C18 : 1ω7c and C19 : 0 cycloω8c as the major fatty acids. The major polar lipids were lipid, aminolipid, phospholipid, phosphatidylcholine, phosphatidylglycerol and phosphatidylethanolamine. The genomic contained a circular chromosome of 5.48 Mb with a DNA G+C content of 65.7 mol%. The genome included 5299 genes. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain J95 T belongs to the genus Ruegeria with highest sequence similarity (96.8 %) to the type strain Ruegeria marina ZH17 T . The combined phenotypic, chemotaxonomic and phylogenetic data suggested that strain J95 T represents a novel species of the genus Ruegeria, for which the name Ruegeria kandeliae sp. nov. is proposed. The type strain is J95 T (=MCCC 1K03284 T =DSM 104293 T ).