Open AccessPaenibacillus elymi sp. nov., isolated from the rhizosphere of Elymus tsukushiensis, a plant native to the Dokdo Islands, Republic of Korea
Author(s) -
Ye-Ji Hwang,
JinSoo Son,
SaYoul Ghim
Publication year - 2018
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.002892
Subject(s) - biology , 16s ribosomal rna , paenibacillus , rhizosphere , phylogenetic tree , botany , elymus , strain (injury) , bacteria , microbiology and biotechnology , gene , poaceae , genetics , anatomy
Strain KUDC6143 T was isolated from the rhizosphere of Elymus tsukushiensis, a plant native to the Dokdo Islands, Republic of Korea. Cells of this bacterial strain were Gram-positive, endospore-forming, motile and rod-shaped. The strain was capable of growth at a temperature of 25-45 °C and at a pH of 6.0-12.0; it showed an optimal growth at a temperature of 30 °C and at a pH of 7.0. In addition, it grew on a tryptic soy agar and in a tryptic soy broth containing less than 4.0 % NaCl (w/v). The cell length ranged from 2.0 to 2.7 µm. KUDC6143 T was catalase-negative and oxidase-positive, and it hydrolysed starch but not casein. Its genomic G+C content was 50.3 mol%. Its major fatty acids were anteiso-C15 : 0, C16 : 0, and iso-C16 : 0. Phylogenetic analysis, based on the 16S rRNA gene sequences, showed that KUDC6143 T belonged to the genus Paenibacillus, with the most closely related type strain being Paenibacillus pinihumi S23 T (97.8 %). Based on its phenotypic characteristics, phylogenetic data and genetic data, strain KUDC6143 T should be considered as representing a novel species of the genus Paenibacillus, for which we propose the name Paenibacillus elymi sp. nov. The type strain is KUDC6143 T (=KCTC 33853 T =DSM 106581 T ).