Open AccessPiscinibacter caeni sp. nov., isolated from activated sludge
Author(s) -
Dong Zhi Chen,
Ning Yu,
Qi Ying Chu,
Jing Chen,
Jie Xu Ye,
Zhuo Cheng,
Shihan Zhang,
Jian Meng Chen
Publication year - 2018
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.002891
Subject(s) - 16s ribosomal rna , biology , putrescine , cadaverine , strain (injury) , phosphatidylglycerol , phylogenetic tree , activated sludge , bacteria , gene , microbiology and biotechnology , biochemistry , phospholipid , wastewater , genetics , enzyme , anatomy , membrane , phosphatidylcholine , engineering , waste management
A yellowish-pigmented bacterial strain, designated as MQ-18 T , was isolated from a sample of activated sludge collected from a pharmaceutical factory in Zhejiang, China. The strain was characterized through a polyphasic taxonomy approach. 16S rRNA gene sequence analysis demonstrated that strain MQ-18 T showed high similarities to Piscinibacter defluvii SH-1 T (99.7 %) and Piscinibacter aquaticus IMCC1728 T (98.4 %), thereby suggesting that it belongs to the genus Piscinibacter. The DNA-DNA relatedness values of this strain to strains SH-1 T and IMCC1728 T were only 35.4 and 33.3 %, respectively. Cells of MQ-18 T were Gram-negative, aerobic, motile, rod-shaped and non-spore forming. This strain exhibited growth at 25-37 °C (optimum: 30 °C) in the presence of 0-3.0 % (w/v) NaCl (optimum, 0 % NaCl) and at pH 5.0-8.0 (pH 7.0). The predominant fatty acids were C12 : 0 (5.5 %), C16 : 0 (33.7 %), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c; 38.5 %), and summed feature 4 (anteiso-C17 : 1 B and/or iso C17 : 1 I; 11.6 %). The main quinone type was ubiquinone-8, and the major polyamines were cadaverine and putrescine. The major polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content was 70.1 mol%. On the basis of its phylogenetic, phenotypic and physiological characteristics, strain MQ-18 T is considered to represent a novel species of the genus Piscinibacter, for which the name Piscinibacter caeni sp. nov. is proposed. The type strain is MQ-18 T (CCTCC AB 2017223 T =JCM 32138 T ).