Open AccessXinfangfangia soli gen. nov., sp. nov., isolated from a diuron-polluted soil
Author(s) -
Qiang Hu,
Long Zhang,
Ping Hang,
Xi-Yi Zhou,
Weibin Jia,
Shunpeng Li,
Jiandong Jiang
Publication year - 2018
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.002887
Subject(s) - biology , 16s ribosomal rna , phylogenetic tree , botany , proteobacteria , bacteria , phylogenetics , genus , strain (injury) , ribosomal rna , microbiology and biotechnology , gene , biochemistry , genetics , anatomy
A novel Gram-staining-negative, non-motile and rod-shaped bacterial strain ZQBW T , which was isolated from a diuron-polluted soil collected near Nanjing, PR China, was investigated for its taxonomic position by a polyphasic approach. ZQBW T grew well at pH 6.0-12.0 (optimum, pH 7.0), 26-35 °C (optimum, 30 °C) and up to 0.5 % NaCl (optimally the absence of NaCl) in R2A broth. The major fatty acids of ZQBW T were C18 : 1ω7c (82.7 %) and C18 : 0 (5.3 %). The polar lipid profile included the major compounds phophatidylcholine, phosphatidylglycerol and phosphatidylmethylethanolamine. The only respiratory quinone was ubiquinone Q-10. The G+C content of genomic DNA was 67.0 mol%. Comparisons with 16S rRNA gene sequences revealed that ZQBW T has the highest sequence similarities with members of the genus Tabrizicola (≤95.97 %), followed by Rhodobacter(≤95.96 %) and Falsirhodobacter(95.95 %) which all belong to the family Rhodobacteraceaein the phylum Proteobacteria. Photosynthesis genes pufLM were not found and photosynthesis pigments were not formed in ZQBW T . On the basis of the results from chemotaxonomic, phenotypic and phylogenetic analysis, ZQBW T represents a novel species of a novel genus, for which the name Xinfangfangia soli gen. nov., sp. nov. is proposed. The type strain is ZQBW T (=KCTC 62102 T =CCTCC AB 2017177 T ).