Open AccessFerrigenium kumadai gen. nov., sp. nov., a microaerophilic iron-oxidizing bacterium isolated from a paddy field soil
Author(s) -
Ashraf Khalifa,
Yuta Nakasuji,
Norikuni Saka,
Hiroki Honjo,
Susumu Asakawa,
Takeshi Watanabe
Publication year - 2018
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.002882
Subject(s) - biology , 16s ribosomal rna , bacteria , strain (injury) , betaproteobacteria , botany , microaerophile , ribosomal rna , microbiology and biotechnology , autotroph , gene , biochemistry , genetics , actinobacteria , anatomy
An iron-oxidizing bacterium, designated strain An22 T , which was isolated from a paddy field soil in Anjo, Japan, was described taxonomically. Strain An22 T was motile by a single polar flagellum, curved-rod, Gram-negative bacterium that was able to grow at 12-37 °C (optimally at 25-30 °C) and at pH 5.2-6.8 (pH 5.9-6.1). The strain grew microaerobically and autotrophically by oxidizing ferrous iron, but did not form stalks, a unique structure of iron oxides. The major cellular fatty acids were C16 : 0 and C16 : 1ω7c/C16 : 1ω6c. The major respiratory quinones were UQ-10 and UQ-8. The strain possessed ribulose-1,5-bisphosphate carboxylase/oxygenase indicating an autotrophic nature via the Calvin-Benson-Bassham cycle. The total DNA G+C content was 61.4 mol%. 16S rRNA gene sequence analysis revealed that strain An22 T was affiliated with the class Betaproteobacteria and clustered with iron-oxidizing bacteria, Gallionella ferrugineaJohan (94.8 % similarity) and Ferriphaselus amnicola OYT1 T (94.4 %) in the family Gallionellaceae. Based on the low 16S rRNA gene sequence similarity to the phylogenetically closest genera and the combination of unique morphological, physiological and biochemical characteristics, strain An22 T represents a novel genus and species within the family Gallionellaceae, for which the name Ferrigenium kumadai gen. nov., sp. nov. is proposed. The type strain is An22 T (=JCM 30584 T =NBRC 112974 T =ATCC TSD-51 T ).