Open AccessSimplicispira suum sp. nov., isolated from a dust collector at a pig farm
Author(s) -
Ha-Young Cho,
Jun Heo,
SeungBeom Hong,
Jeong-Seon Kim,
Soon-Wo Kwon,
Soojin Kim
Publication year - 2018
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.002874
Subject(s) - 16s ribosomal rna , biology , phosphatidylglycerol , strain (injury) , phylogenetic tree , microbiology and biotechnology , bacteria , agar plate , gene , biochemistry , genetics , phospholipid , anatomy , membrane , phosphatidylcholine
A novel Gram-stain-negative, strictly aerobic, polar-flagellated and rod-shaped bacterium, designated SC1-8 T , was isolated from a dust collector at a pig farm located in Wanju-gun, Jeollabuk-do, Republic of Korea. The strain grew within a temperature range of 4-37 °C (optimum, 28-30 °C), at pH 7.0-9.0 (pH 7.0-8.0) and with 0-2 % (w/v) NaCl (0 %). Colonies were white-beige, circular and convex after 4 days of incubation on Reasoner's 2A agar. Based on the 16S rRNA gene sequence analysis, strain SC1-8 T was a member of the genus Simplicispira, revealing the highest sequence similarities to Simplicispira limi EMB325 T (97.9 %), Simplicispira psychrophila DSM 11588 T (97.4 %), Acidovorax defluvii BSB411 T (97.3 %), Simplicispira piscis RSG39 T (97.1 %) and Simplicispira metamorpha DSM 1837 T (97.0 %). The predominant respiratory quinone was Q-8. The polar lipids were phosphatidylethanolamone, diphosphatidylglycerol and phosphatidylglycerol. The major fatty acids (>10 % of the total fatty acids) were composed of C16 : 0 and summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c). The DNA G+C content was 63.3 mol%. On the basis of phenotypic, genotypic and phylogenetic evidence, strain SC1-8 T is presented as a novel species, for which the name Simplicispira suum sp. nov. is proposed. The type strain is SC1-8 T (=KACC 19329 T =NBRC 113111 T ).