Open AccessSolimonas fluminis sp. nov., isolated from a freshwater river
Author(s) -
Yunho Lee,
Boeun Lee,
Kangseok Lee,
Che Ok Jeon
Publication year - 2018
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.002865
Subject(s) - biology , 16s ribosomal rna , phylogenetic tree , strain (injury) , microbiology and biotechnology , phylogenetics , genus , flagellum , ribosomal rna , bacteria , botany , gene , genetics , anatomy
A strictly aerobic, catalase-negative and oxidase-positive bacterium (HR-BB T ), isolated from a water sample of the Han River, was taxonomically studied using a polyphasic approach. Cells were Gram-stain-negative motile rods with a polar flagellum. The strain grew at 20-35 °C and pH 7-8 and in the absence of NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain HR-BB T belonged to the family Nevskiaceae in the phylum Proteobacteria and formed a phylogenic lineage with members of the genus Solimonas. A comparison of the 16S rRNA gene sequences of strain HR-BB T and the type strains of closely related species of the genus Solimonas showed that it shared highest sequence similarity with Solimonas terrae KIS83-12 T (94.9 %), Solimonas soli DCY12 T (94.8 %), Solimonas variicoloris MN28 T (94.4 %) and Solimonas flava CW-KD 4 T (94.2 %). The fatty acids of the strain consisted of summed features 8 (comprising C18 : 1ω6c and/or C18 : 1ω7c) and 3 (comprising C16 : 1ω6c and/or C16 : 1ω7c), C16 : 0 and C12 : 0 as major components. The polar lipids comprised phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, three unidentified phospholipids and an unidentified lipid. Ubiquinone-8 was detected as the sole respiratory quinone. The DNA G+C content of strain HR-BB T was 68.5 mol%. Based on the genotypic, chemotaxonomic and phenotypic analyses, strain HR-BB T represents a novel species of the genus Solimonas, for which the name Solimonas fluminis sp. nov. is proposed. The type strain is HR-BB T (=KACC 19410 T =JCM 32268 T ).