Open AccessNocardioides allogilvus sp. nov., a novel actinobacterium isolated from a karst cave
Author(s) -
Lingyu Zhang,
Ming Hong,
Zhuo-Li Zhao,
Wei-Li Ji,
Nimaichand Salam,
JianYu Jiao,
BaoZhu Fang,
Wenjun Li,
Guoxing Nie
Publication year - 2018
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.002863
Subject(s) - biology , 16s ribosomal rna , microbiology and biotechnology , strain (injury) , peptidoglycan , diamino acid , rhamnose , diaminopimelic acid , halophile , cell wall , bacteria , botany , galactose , biochemistry , gene sequence , genetics , anatomy
A novel actinobacterium, designated strain CFH 30205 T , was isolated from a soil sample collected from a karst cave in Luoyang, Henan Province, China. The taxonomic position of the strain was investigated by using a polyphasic approach. Cells of the strain were aerobic, Gram-stain-positive, non-motile and rod-shaped. The strain was found to be catalase- and oxidase-positive. Strain CFH 30205 T grew optimally at 28 °C, pH 9.0 and in the presence of up to 1.5 % NaCl (w/v). On the basis of 16S rRNA gene sequence analysis, strain CFH 30205 T was most closely related to the type strains of Nocardioides terrigena DS-17 T (97.6 % sequence similarity) and Nocardioides sediminis MSL-01 T (97.0 %). The DNA G+C content was determined to be 69.9 mol%. ll-2,6-Diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan. The whole-cell sugars were mannose, xylose and galactose. The major isoprenoid quinone was MK-8 (H4), and the major fatty acids (>10 %) were iso-C16 : 0 and anteiso-C14 : 0. The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unidentified phospholipid. On the basis of phenotypic, genotypic and phylogenetic data, strain CFH 30205 T merits representation of a novel species of the genus Nocardioides, for which the name Nocardioides allogilvus sp. nov. is proposed. The type strain is CFH 30205 T (=KCTC 49020 T =CGMCC 4.7457 T ).