Open AccessSolimicrobium silvestre gen. nov., sp. nov., isolated from alpine forest soil
Author(s) -
Rosa Margesin,
Luciana Albuquerque,
De-Chao Zhang,
Hugo J.C. Froufe,
Rita Severino,
Inês Roxo,
Conceição Egas,
Milton S. da Costa
Publication year - 2018
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.002861
Subject(s) - biology , 16s ribosomal rna , phylogenetic tree , botany , microbiology and biotechnology , strain (injury) , ribosomal rna , phylogenetics , bacteria , gene , biochemistry , genetics , anatomy
A Gram-stain-negative, rod-shaped, motile, catalase and cytochrome c oxidase-positive bacterial strain, designated S20-91 T , was isolated from alpine forest soil. Growth occurred within a temperature range of 0-25 °C. Yeast extract was required for growth. Phylogenetic analysis based on 16S rRNA gene sequencing showed that strain S20-91 T was related to the genus Herminiimonas and had the highest 16S rRNA gene sequence similarity to Herminiimonas arsenicoxydans ULPAs1 T (96.5 %). The strain contained ubiquinone 8 as the predominant respiratory quinone and phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol as the major polar lipids. The major cellular fatty acids (>10 %) were C16 : 1ω7c (55.3 %) and C16 : 0 (25.6 %). The genomic DNA G+C content was 47.6 mol%. Combined data of genomic, phylogenetic, phenotypic and chemotaxonomic analyses demonstrated that strain S20-91 T represents a novel genus and species, for which the name Solimicrobium silvestre gen. nov., sp. nov. is proposed. The type strain is S20-91 T (=DSM 104733 T =LMG 30010).