Open AccessLactobacillus bambusae sp. nov., isolated from traditional fermented ma bamboo shoots in Taiwan
Author(s) -
Jia-Rong Guu,
Liting Wang,
Moriyuki Hamada,
Chihuei Wang,
Ru-Wei Lin,
Lina Huang,
Koichi Watanabe
Publication year - 2018
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.002837
Subject(s) - biology , lactobacillus , 16s ribosomal rna , strain (injury) , botany , microbiology and biotechnology , bacteria , fermentation , food science , genetics , anatomy
A Gram-stain-positive strain, BS-W1 T , was isolated from a traditional fermented ma bamboo shoots (Dendrocalamus latiflorus Munro) product of Taiwan. It was rod-shaped, non-motile, non-haemolytic, asporogenous, facultatively anaerobic, heterofermentative and did not exhibit catalase or oxidase activities. Comparative analysis of 16S rRNA, pheS, rpoA and gyrB gene sequences demonstrated that the novel strain BS-W1 T was a member of the genus Lactobacillus. On the basis of 16S RNA gene sequence similarity, the type strains of Lactobacillus oryzae (94.4 % similarity), Lactobacillus acidifarinae (93.8 %), Lactobacillus namurensis (93.7 %) and Lactobacillus zymae (93.7 %) were the closest neighbours to strain BS-W1 T . The pheS, rpoA and gyrB gene sequence similarities of strain BS-W1 T to closely related these species were less than 80.2 %. DNA-DNA reassociation values with these type strains were 21.0-33.8 %. The DNA G+C content was 46.6 mol%. The average nucleotide identity values between BS-W1 T and the closest relatives were lower than 70 %. Phenotypic and genotypic features demonstrated that the strain represents a novel species of the genus Lactobacillus, for which the name Lactobacillus bambusae sp. nov. is proposed. The type strain is BS-W1 T (=BCRC 80970 T =NBRC 112377 T ).