Open AccessChitinophaga caeni sp. nov., isolated from activated sludge
Author(s) -
Decai Jin,
Xiao Kong,
Jie Wang,
Jiajun Sun,
Xiangyang Yu,
Xuliang Zhuang,
Ye Deng
Publication year - 2018
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.002811
Subject(s) - biology , 16s ribosomal rna , phylogenetic tree , strain (injury) , bacteroidetes , microbiology and biotechnology , genomic dna , gene , bacteria , genome size , genome , diamino acid , phylogenetics , genetics , gene sequence , anatomy
A novel Gram-stain-negative, non-motile, non-spore-forming, rod-shaped, strictly aerobic bacterium, designated strain 13 T , was isolated from an activated sludge wastewater treatment plant in Beijing, China. 16S rRNA gene sequence analysis placed this organism within the genus Chitinophaga of the class Bacteroidetes, with Chitinophaga skermanii CC-SG1B (92.4 % gene sequence similarity) and Chitinophaga rupis CS5-B1 (91.2 %) as its closest relatives. This isolate contained meso-diaminopimelic acid as the diagnostic diamino acid and the whole-cell hydrolysate was ribose. Phosphatidylethanolamine was the predominant polar lipid. The major cellular fatty acids were iso-C15 : 0, C16 : 1ω5c and iso-C17 : 0 3-OH. Menaquinone-7 was the only isoprenoid quinone present. The complete genome of the novel strain was sequenced; the size of the genome was 5.28 Mb and the genomic DNA G+C content was 42.5 mol%. The phenotypic, chemotaxonomic and phylogenetic data showed that strain 13 T represents a novel species of the genus Chitinophaga, for which the name Chitinophaga caeni sp. nov. is proposed. The type strain is 13 T (=CICC 24262 T =KCTC 62265 T ).