Open AccessZhouia spongiae sp. nov., isolated from a marine sponge
Author(s) -
Lingping Zhuang,
Borong Lin,
Fei Qin,
Lianzhong Luo
Publication year - 2018
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.002808
Subject(s) - sponge , biology , botany
A taxonomic study was carried out on strain HN-Y44 T , which was isolated from sponge collected from Yangpu Bay, Hainan, China. Cells of strain HN-Y44 T were Gram-stain-negative, non-motile, rod-shaped, yellow-pigmented and grew at 10-40 °C (optimum, 28 °C), at pH 6-10 (optimum, 7-8) and in 0-8 % (w/v) NaCl (optimum, 2-5 %). This isolate was positive for nitrate reduction, denitrification, oxidase, catalase and aesculin hydrolysis, but negative for indole production and urease. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain HN-Y44 T belongs to the genus Zhouia and is clearly distinct from the other described species of this genus, Zhouia amylolytica, with a 16S rRNA gene sequence similarity of 96.85 %. The dominant fatty acids were iso-C15 : 0, iso-C15 : 1 G and iso-C17 : 0 3-OH. The major polar lipids comprised phosphatidylethanolamine, four unidentified aminolipids, four unidentified phospholipids and one unidentified lipid. The respiratory lipoquinone was identified as MK-6. The G+C content of the genomic DNA was 32.9 mol%. On the basis of the phenotypic and phylogenetic data, strain HN-Y44 T represents a novel species of the genus Zhouia, for which the name Zhouia spongiae sp. nov. is proposed, with the type strain HN-Y44 T (=MCCC 1K03329 T =LMG 30460 T ).