Open AccessStreptomyces aqsuensis sp. nov., isolated from ditch sediment in Xinjiang, China
Author(s) -
Jianming Wang,
Qiaoyan Zhang,
Yang Wang,
Song Qin,
Xiaoxia Luo
Publication year - 2018
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.002804
Subject(s) - biology , ditch , china , sediment , ecology , archaeology , paleontology , history
A novel actinobacterium, designated TRM46399 T , was isolated from ditch sediment in Xinjiang Province, Northwest China. Phylogenetic, physiological, chemotaxonomic and morphological analyses demonstrated that strain TRM46399 T belonged to the genus Streptomyces. The strain was aerobic, Gram-stain-positive, had aerial mycelia that branched monopodially and formed chains of arthrospores. The spores were oval to cylindrical with smooth surfaces. The strain had an optimum NaCl concentration for growth of 5 % (w/v). The whole-cell sugar pattern of TRM46399 T consisted of ribose, xylose, mannose and galactose. The predominant menaquinones were MK-9(H6), MK-9(H8) and MK-9(H10). The polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannosides. Major fatty acids were iso-C16 : 0 (47.8 %), iso-C14 : 0 (13.5 %), C16 : 0 (8.9 %), anteiso-C15 : 0 (8.4 %), anteiso-C17 : 0 (4.2 %) and C17 : 0cyclo (2.7 %). The G+C content of the genomic DNA was 68.7 mol%. Comparative 16S rRNA gene sequence analysis indicated that strain TRM46399 T (KY688182) was phylogenetically most closely related to Streptomyces xinghaiensis S187 T (99.13 % sequence similarity); however, DNA-DNA hybridization studies between stain TRM46399 T and S. xinhaiensis S187 T showed only 65.32 % relatedness. Based on the evidence from this polyphasic study, strain TRM46399 T represents a novel species of the genus Streptomyces, for which the name Streptomyces aqsuensis sp. nov. is proposed. The type strain is TRM46399 T (=KCTC 39610 T =CCTCC AA 2015009 T ).