Open AccessPannonibacter carbonis sp. nov., isolated from coal mine water
Author(s) -
Lijun Xi,
Nenghu Qiao,
Dejian Liu,
Jing Li,
Jingjing Zhang,
Jianguo Liu
Publication year - 2018
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.002794
Subject(s) - biology , 16s ribosomal rna , genomic dna , oxidase test , phylogenetic tree , bacteria , strain (injury) , microbiology and biotechnology , ribosomal rna , dna , gene , biochemistry , enzyme , genetics , anatomy
Two bacterial strains were isolated from coal mine water in China. Isolates were facultatively anaerobic, Gram-stain-negative, rod-shaped, motile by means of a single polar flagellum, and they did not produce bacteriochlorophyll α. Cells grew in tryptic soy broth with 0-5.5 % (w/v) NaCl, at 4-55 °C and pH 3.5-10.5. Isolates were positive for catalase, oxidase, urease, Voges-Proskauer test, gelatin hydrolysis and H2S production. Analysis of 16S rRNA gene sequences indicated that the closest relatives of strains Q4.6 T and Q2.11 were the type strains Labrenzia suaedae DSM 22153 T (97.4 %), Pannonibacter phragmitetus DSM 14782 T (96.9 and 97.0 %) and Pannonibacter indicus DSM 23407 T (96.8 %). The genomic average nucleotide identity (ANI) value for Q4.6 T and Q2.11 was 100 %; however, this value was less than 77.7 % for the type strains P. phragmitetus and P. indicus, and less than 74.0 % for the type strain L. suaedae. The cellular fatty acid profile of strains Q4.6 T and Q2.11 consisted primarily of C18 : 1ω7c. The principal quinone of the isolates was Q-10. The polar lipid profile consisted of diphosphatidyl glycerol, phosphatidyl glycerol, phosphatidyl ethanolamine and phosphatidyl choline. On the basis of phylogenetic analysis, genomic ANI analysis, DNA-DNA hybridization results, as well as phenotypic and chemotaxonomic data, strains Q4.6 T and Q2.11 are assigned as a novel species within the genus Pannonibacter. The type strain is Pannonibacter carbonis Q4.6 T (=CGMCC 1.15703 T =KCTC 52466 T ).