Open AccessFluviicola kyonggii sp. nov., a bacterium isolated from forest soil and emended description of the genus Fluviicola
Author(s) -
Ram Hari Dahal,
Jaisoo Kim
Publication year - 2018
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.002759
Subject(s) - 16s ribosomal rna , biology , phylogenetic tree , bacteria , strain (injury) , microbiology and biotechnology , bacteroidetes , botany , gene , biochemistry , genetics , anatomy
A novel bacterium, designated strain CA-1 T , was isolated from forest soil in Kyonggi University. Cells were strictly aerobic, Gram-stain-negative, catalase-positive, oxidase-negative, non-motile, non-spore-forming, rod-shaped and red-orange-pigmented. Strain CA-1 T hydrolysed casein and DNA. It was able to grow at 15-37 °C, pH 5.5-9.0 and at 0-2 % (w/v) NaCl concentration. Flexirubin-type pigments were present. Phylogenetic analysis based on its 16S rRNA gene sequence indicated that strain CA-1 T formed a lineage within the family Crocinitomicaceae of the phylum Bacteroidetes that was distinct from Fluviicola hefeinensis MYL-8 T (96.8 % sequence similarity) and Fluviicola taffensis DSM 16823 T (96.1 %). Strain CA-1 T contained menaquinone-6 as a sole respiratory quinone. The major polar lipids were phosphatidylethanolamine, unidentified aminolipids, an unidentified aminophospholipid and an unidentified lipid. The major cellular fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH, C15 : 0 2-OH, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and iso-C15 : 1 G. The DNA G+C content of strain CA-1 T was 44.1 mol%. The polyphasic characterization revealed that strain CA-1 T represents a novel species in the genus Fluviicola, for which the name Fluviicola kyonggii sp. nov. is proposed. The type strain is CA-1 T (=KEMB 9005-526 T =KACC 19148 T =NBRC 112684 T ).