Open AccessSubsaxibacter sediminis sp. nov., isolated from Arctic glacial sediment and emended description of the genus Subsaxibacter
Author(s) -
Avinash Sharma,
Kunal Jani,
Guang-Da Feng,
Prachi Karodi,
Venkata Ramana Vemuluri,
Honghui Zhu,
Sisinthy Shivaji,
Vishal Thite,
Swapnil Kajale,
Praveen Rahi,
Yogesh S. Shouche
Publication year - 2018
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.002729
Subject(s) - biology , genus , sediment , arctic , zoology , glacial period , ecology , microbiology and biotechnology , paleontology
A Gram-stain-negative, yellowish-orange pigmented, rod-shaped, motile bacterium, designated strain ARC111 T , was isolated from sediment of Arctic permafrost at Midtre Lovénbreen glacier, Svalbard. 16S rRNA gene based identification of strain ARC111 T demonstrated highest sequence similarities to Subsaxibacter broadyi P7 T (97.8 %) and Subsaxibacter arcticus JCM30334 T (97.5 %) and ≤95.2 % with all other members of the family Flavobacteriaceae. Phylogenetic analysis revealed the distinct positioning of strain ARC111 T within the genus Subsaxibacter. The G+C content of ARC111 T was 37.8±0.5 mol% while DNA-DNA hybridization depicted 35.6 % relatedness with S. arcticus JCM30334 T . Strain ARC111 T had C15 : 0iso, C16 : 0iso 3-OH, C15 : 1iso G, C15 : 0anteiso, C16 : 1iso H and C17 : 0iso 3-OH as major (>5 % of the total) cellular fatty acids and MK-6 was the predominant respiratory quinone. The polar lipid profile of strain ARC111 T consisted of phosphatidylethanolamine, aminolipid and an unidentified lipid. Strain ARC111 T harboured sym-homospermidine as the major polyamine. Characteristic differences obtained using polyphasic analysis of strain ARC111 T and its closest relatives suggested that strain ARC111 T is a novel species of genus Subsaxibacter, for which the name Subsaxibacter sediminis sp. nov. has been proposed. The type strain is ARC111 T (=MCC 3191 T =KCTC 42965 T =LMG 29783 T =GDMCC 1.1201 T ).