Bizionia berychis sp. nov., isolated from intestinal tract of a splendid alfonsino (Beryx splendens)

A Gram-stain-negative, non-motile, aerobic and rod-shaped bacterial strain, designated RA3-3-1 T , was isolated from splendid alfonsino (Beryxsplendens) collected from the North Pacific Ocean. Strain RA3-3-1 T grew optimally at 25 °C, at pH 7.0-8.0 and in the presence of 1.0-3.0 % (w/v) NaCl. The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain RA3-3-1 T belonged to the genus Bizionia, clustering with the type strain of Bizionia fulviae. Strain RA3-3-1 T exhibited 16S rRNA gene sequence similarities of 98.7, 97.6 and 97.3 % to the type strains of B. fulviae, Bizionia paragorgiae and Bizionia saleffrena, respectively, and of 95.5-96.4 % to the type strains of the other Bizionia species. Strain RA3-3-1 T contained MK-6 as the predominant menaquinone and anteiso-C15 : 0, iso-C16 : 0 3-OH, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), iso-C17 : 0 3-OH, iso-C15 : 0 and C17 : 0 2-OH as the major fatty acids. The major polar lipids detected in strain RA3-3-1 T were phosphatidylethanolamine, one unidentified lipid and one unidentified aminolipid. The DNA G+C content of strain RA3-3-1 T was 34.1 mol% and its DNA-DNA relatedness values with the type strains of B. fulviae, B. paragorgiae and B. saleffrena were 12-29 %. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain RA3-3-1 T is separate from recognized species of the genus Bizionia. On the basis of the data presented, strain RA3-3-1 T is considered to represent a novel species of the genus Bizionia, for which the name Bizionia berychis sp. nov. is proposed. The type strain is RA3-3-1 T (=KCTC 62140 T =NBRC 113024 T ).