Open AccessVitellibacter todarodis sp. nov., isolated from intestinal tract of a squid (Todarodes pacificus)
Author(s) -
Hyun-Chul Kim,
YoungOk Kim,
Sooyeon Park,
BoHye Nam,
Dong-Gyun Kim,
Jimin Park,
JungHoon Yoon
Publication year - 2018
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.002655
Subject(s) - biology , 16s ribosomal rna , strain (injury) , phylogenetic tree , genus , bacteria , microbiology and biotechnology , phylogenetics , gene , zoology , anatomy , genetics
A Gram-stain-negative, non-motile, aerobic and rod-shaped or ovoid bacterial strain, designated MYP2-2 T , was isolated from the intestinal tract of a squid (Todarodes pacificus) collected from the East Sea, South Korea, and subjected to a polyphasic taxonomic study. Strain MYP2-2 T grew optimally at 30-35 °C and in the presence of 2.0 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain MYP2-2 T belonged to the genus Vitellibacter. Strain MYP2-2 T exhibited 16S rRNA gene sequence similarities of 95.4-96.6 % to the type strains of Vitellibacter species and of less than 94.5 % to the type strains of other recognized species examined. Strain MYP2-2 T contained menaquinone MK-6 as the predominant respiratory quinone and iso-C15 : 0 and iso-C17 : 0 3-OH as the major fatty acids. The major polar lipids detected in strain MYP2-2 T were phosphatidylethanolamine and one unidentified lipid. The DNA G+C content of strain MYP2-2 T was 41.6 mol%. Differential phenotypic properties, together with its phylogenetic distinctiveness, revealed that strain MYP2-2 T is separated from recognized species of the genus Vitellibacter. On the basis of the data presented, strain MYP2-2 T is considered to represent a novel species of the genus Vitellibacter, for which the name Vitellibacter todarodis sp. nov. is proposed. The type strain is MYP2-2 T (=KCTC 62141 T =NBRC 113025 T ).