Open AccessStackebrandtia soli sp. nov., a novel actinobacterium isolated from a soil sample
Author(s) -
Min-Jiao Liu,
Chun-Zhi Jin,
Dong-Jin Park,
Mipeshwaree Devi Asem,
Min Xiao,
Nimaichand Salam,
Wenjun Li,
ChangJin Kim
Publication year - 2018
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.002654
Subject(s) - biology , 16s ribosomal rna , phylogenetic tree , strain (injury) , diamino acid , glycolipid , diaminopimelic acid , botany , genomic dna , xylose , microbiology and biotechnology , galactose , biochemistry , fermentation , gene , gene sequence , anatomy
An aerobic actinobacterium, strain AN130378 T , was isolated from a soil sample collected in Korea and subjected to taxonomic investigation using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequence data showed that strain AN130378 T is a member of the genus Stackebrandtia, with sequence similarities of 97.3 % to Stackebrandtia albiflava YIM 45751 T and 97.1 % to Stackebrandtia endophytica YIM 64602 T . The whole-cell hydrolysates contained meso-diaminopimelic acid as the diagnostic diamino acid, and galactose, glucose and xylose. The major menaquinones were MK-11(H4), MK-10(H4) and MK-11(H6), while the major fatty acids were identified as iso-C15 : 0, anteiso-C17 : 0, anteiso-C15 : 0, iso-C17 : 0 and summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c). The polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylmethylethanolamine, an unidentified aminophospholipid, four unidentified glycolipids, three unidentified phospholipids and two unidentified polar lipids. The G+C content of the genomic DNA was determined to be 67.7 mol%. All chemotaxonomic and genotypic data indicated that the strain belongs to the genus Stackebrandtia. On the basis of morphological, chemotaxonomic data and phylogenetic analysis, strain AN130378 T is considered to represent a novel species within the genus Stackebrandtia, for which the name Stackebrandtia soli sp. nov. is proposed. The type strain is AN130378 T (=DSM 103573 T =KCTC 39809 T ).